Modifying Enzymes
Bulldog Bio Inc PNGase F PRIME Glycosidase 500UL
LD: Manufactured by N-Zyme Scientifics and supplied by Bulldog Bio Inc. Reagent; Molecular Biology; PNGase F PRIME, Glycosidase Specific for N-linked Oligosaccharides (Mannose, Hybrid, and Complex), 500 units/ul, 2x 50ul vials, 50,000 IUB milliunts
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 10000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
Enzo Life Sciences MMP-9 (catalytic domain) (human), (recombinant, E. coli) (10 µg)
Alternative name: Matrix metalloproteinase 9. Gelatinase B. 92 kDa Type IV collagenase. MW: 39 kDa. Purity: ≥95% (SDS-PAGE). Formulation: Liquid. In 50mM TRIS, pH 7.5, containing 1mM calcium chloride, 300mM sodium chloride, 5μM zinc chloride, 0.1% Brij-35 and 15% glycerol. Source: Produced in E. coli. Active recombinant matrix metalloproteinase-9 (MMP-9, gelatinase B, 92 kDa type IV collagenase) cloned from human cDNA. The enzyme consists of residues Phe107-Pro449 (NM_004994), which comprises the catalytic/fibronectin domain of human MMP-9, with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-9 which lacks the C-terminal hemopexin domain. Activity toward its targets, such as gelatin. CASein, or peptide substrates, is unaffected. UniProt: P14780. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. Long Term Storage: -80°C
New England Biolabs, Inc. T7 RNA Polymerase – 25000 units
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. T4 RNA Ligase 1 (ssRNA Ligase) – 5000 units
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3' - 5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
BioVendor, LLC PROTEIN LYSOSTAPHIN 5 MG
TypeRecombinant proteinDescriptionLysostaphin, an endopeptidase specific for the cell wall peptidoglycan of staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies and for the preparation of protoplasts for transformation. Preparation and analysis of bacterial DNA has become a powerful tool used by clinical and other microbiologists in epidemiological studies aimed at tracing sources of infection or bacterial contamination.SourceE. coliPurity97.5% as determined by RP-HPLC.Biological ActivityDetermined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH8.0, 30°C.FormulationThe protein was lyophilized without any additives.Physical AppearanceSterile filtered white lyophilized (freeze-dried) powder.
New England Biolabs, Inc. Cre Recombinase – 250 units
Cre Recombinase is a Type I topoisomerase from bacteriophage P1 that catalyzes the site-specific recombination of DNA between loxP sites. The enzyme requires no energy cofactors and Cre-mediated recombination quickly reaches equilibrium between substrate and reaction products. The loxP recognition element is a 34 base pair (bp) sequence comprised of two 13 bp inverted repeats flanking an 8 bp spacer region which confers directionality. Recombination products depend on the location and relative orientation of the loxP sites. Two DNA species containing single loxP sites will be fused. DNA between directly repeated loxP sites will be excised in circular form while DNA between opposing loxP sites will be inverted with respect to external sequences.
American Research Products Inc GENIEPURE WATER DNASE RNASE FR
5000265171 GENIEPURE WATER DNASE RNASE FR
Active Motif Preindex Tn5 Transposomes 1x96
Pre-indexed Assembled Tn5 Transposomes are provided in a flexible breakaway 96-well plate format to enable higher throughput ATAC-Seq assays. Each well contains a transposome loaded with a unique i5 and i7 index, enabling direct indexing during tagmentation. By having a unique i7 & i5 Indexed Primer combination in each well, it is easy to perform multiple ATAC-Seq reactions without having to pipette primers separately and keep track of the combinations.
GENOVIS INC GalactEXO, 2000 units
GalactEXO is a Beta-galactosidase for efficient hydrolysis of galactose.
Discovery Life Sciences SUPERSOMES ULTRA HM AOX1 5MG
Corning Supersomes Ultra Human Aldehyde Oxidase (AOX1), 5 mg/mL, 0.5 mL
Cosmo Bio Usa Inc L GLUTAMATE OXIDASE 25 RECOMB
Recombinant L-Glutamate Oxidase (EC 1.4.3.11) produced in E. Coli. Lyophilized. 25 units/vial. Does not react with glutamine, aspartate, D-glutamate or 16 other L-amino acids.
New England Biolabs, Inc. USER® Enzyme – 50 units
USER (Uracil-Specific Excision Reagent) Enzyme generates a single nucleotide gap at the location of a uracil. USER Enzyme is a mixture of Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease VIII. UDG catalyses the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3 and 5 sides of the abasic site so that base-free deoxyribose is released.Applications of USER include:
- Directional RNA Seq
- NEBNext adaptor cleavage
- USER cloning
New England Biolabs, Inc. T4 Polynucleotide Kinase Reaction Buffer – 4 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. Taq DNA Ligase – 10000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).