Modifying Enzymes
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 2000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Endonuclease VIII – 5000 units
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves 3' and 5' to the AP site leaving a 5' phosphate and a 3' phosphate. Damaged bases recognized and removed by Endonuclease VIII include urea, 5, 6- dihydroxythymine, thymine glycol, 5-hydroxy-5- methylhydantoin, uracil glycol, 6-hydroxy-5, 6-dihydrothymine and methyltartronylurea. While Endonuclease VIII is similar to Endonuclease III, Endonuclease VIII has and lyase activity while Endonuclease III has only lyase activity.
New England Biolabs, Inc. HpaII Methyltransferase – 100 units
HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C5) of the sequence CCGG.
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
New England Biolabs, Inc. Endoglycosidase Reaction Buffer Pack – 4 x 1 ml
The Endoglycosidase Reaction Buffer Pack contains four buffers: 10X Glycobuffer 2, 10X Glycobuffer 3, 10X Glycoprotein Denaturing Buffer and 10% NP-40, most commonly used in endoglycosidase reactions.
Bioassay Systems QuantiChrom™ Pectinase Assay Kit. For quantitative determination of pectinase activity determination in biological samples.
For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.
Bioworld Lysozyme, 10 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected
STEMCELL Technologies DNase I, ACF, Size: 100000 U
Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase with Buffer, 2,500 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures
New England Biolabs, Inc. PspXI – 200 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
New England Biolabs, Inc. T5 Exonuclease – 1000 units
T5 Exonuclease degrades DNA in the 5 to 3 direction. T5 Exonuclease is able to initiate nucleotide removal from the 5 termini or at gaps and nicks of linear or circular dsDNA. However, the enzyme does not degrade supercoiled dsDNA. T5 Exonuclease also has ssDNA endonuclease activity.
New England Biolabs, Inc. Thermostable USER III Enzyme - 50 units
Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil. Thermostable USER III Enzyme is a mixture of Afu Uracil DNA glycosylase (UDG) and the DNA glycosylase-lyase Endonuclease IV. Afu UDG catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3’ and 5’ sides of the abasic site so that base-free deoxyribose is released leaving a 5’ deoxyribose phosphate (dRP) and a 3’-hydroxyl. Thermostable USER III Enzyme also has 3’-diesterase activity which can remove 3’ phosphate, 3’-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups. Thermostable USER III Enzyme is optimally active at 65C.
Bioworld Pectinase, 10 g
Pectinase Also known as Poly-(1,4-a-D-galacturonide)glycanohydrolase. Used in plant protoplast preparation to digest cell wall prior to organelle isolation.
LGC GENOMICS LLC (Lucigen) Riboguard RNase Inhibitor, 2500 units @ 40 U/LRNA is precious. Protect it with this high quality, effective RNase inhibitor. Manufactured under ISO-13485.
RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.